Wei Deng

3 Questions 8 Answers 0 Followers

Questions related from Wei Deng

Does any one have experience using Western blotting to measure IC50 in cell based phosphorylation assay?

I am trying to measure the IC50 of a kinase inhibitor in cells. The approach I am using now is Western blotting. However, I have a lot variations from experiment to experiment and with/without...

03 March 2015 4,843 6 View

Does anyone have a good marker for hilar interneurons in the hippocampus?

I am looking for a good antibody to label hilar interneurons in the hippocampus. I have tried several antibodies against GABA and GAD from millipore but did not get good signals. Can someone...

09 September 2014 2,019 7 View

CFos antibody for immunofluorescent staining?

We are having trouble of cFos antibodies from our original sources (Santa Cruz and EMD Millipore) for IF staining of mouse brain tissue. I am wondering if anybody has experiences of using cFos...

08 August 2014 8,733 3 View