Do GFP or DiI "leak" from cells following fixation with 4% PFA? What rate will they be lost?

Hi all, I am looking to measure proliferation rate using DiI (red), a lipophilic dye. The cells I am using are A549-H2B-GFP cells that stably express GFP. I stain the A549-H2B-GFP cells with...

09 March 2012 7,926 2 View