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if A136 is growing well n LB it mean it is active, then why it is not growing in AT media? can anybody recommend some changes in At media specification?
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why we use 0.85% Nacl in 50% glycerol for storage of bacteria at -80?
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Is it possible that A. tumefaciens A136 encounter some type of Mutation after serial culturing? if not then why it's not giving positive result even for positive control in Quorum sensing...
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