Wangsheng Ji

2 Questions 5 Answers 0 Followers

Questions related from Wangsheng Ji

How to introduce a single point mutation in cell lines by CRISPR-Cas9 method ?

Hi everyone. I have experience using the CRISPR-Cas9 system to establish knockout cell lines. Now, I am planning to introduce a single point mutation into my gene of interest using the CRISPR...

05 April 2023 8,685 4 View

Do I need to add calcium to the medium when stimulating jurkat with PMA and ionomycin?

I want to stimulate jurkat cells with PMA and ionomycin. Whether it is necessary to add calcium to the medium. if needed, How much concentration should I use?

11 December 2017 2,646 4 View