Wanda Nowak

3 Questions 3 Answers 0 Followers

Questions related from Wanda Nowak

How can I isolate RNA from primary embryonic neurons?

I need to isolate RNA from primary embryonic cortical neurons at different time points. I bought the cells from Life Technologies and plated them in a 48-well plate according to the company's...

29 January 2016 5,168 2 View

How can I transiently transfect U251 cells?

I need to transfect U251 and I've been using Lipofectamine 2000 with poor efficiency (less than 30%). Can anyone provide me a good transfection protocol for this cell line?

12 August 2015 7,444 5 View

How do I re-select stable cell lines?

I transfected U251 cells, selected them with blastacidin (20 ug/ml) for 2 weeks and check the expression of the product by qPCR and western. Although the mRNA levels are quite good, I have very...

29 July 2015 5,584 4 View