Wan Ling Wong

5 Questions 2 Answers 0 Followers

Questions related from Wan Ling Wong

Why MALDI observed size 3% larger than expected size?

Hi all, MALDI analysing my protein gave a MW 3% larger than the calculated size. Does anyone know why is that? I tried checking charges but still couldn't come up with a reasonable explanation....

01 January 2019 6,420 2 View

What is the ionisation enthalpy of urea? Can urea be used as buffer for ITC experiments?

Hi all, I have an unhappy protein. I managed to take it down to 2M urea and previous studies have shown that it is refolded in 4M urea. So I am happy with the protein staying in 2M urea. The...

01 January 2018 9,929 6 View

Why my negative control of my PCR shows a band?

Hi, I have done cloning for years and just recently I have encountered this problem. When I do my PCR screening with Taq, I never run along a negative control because I know what size I am...

06 June 2016 3,512 7 View

Which technique should I use to observe nanostructures?

Hi, I am engineering nanocages and it will be approx. 3.1nm in diameter and approx. 15.3nm in length. My colleague tried using TEM but it is at its limit. I am open to suggestion. Thank...

05 May 2016 5,581 3 View

How GST is different from SUMO in terms of being partner protein in protein expression and purification?

Hi! I have tried to expressed my recombinant protein with and without GST. Without GST, low expression and cannot be purified; with GST, I could not get a clean separation and it did not help...

04 April 2016 6,789 3 View