Waleed Fawzy Abdelaziz Marei

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Questions related from Waleed Fawzy Abdelaziz Marei

DCF staining in oocytes for oxidative stress. Why does the staining intensity continuously increase under the fluorescence microscope?

While visualizing the green fluorescence intensity of oocytes stained with DCF staining, we notice that the intensity continuously and rapidly increase once you start the excitation. Any idea why...

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How specific do you think is your siRNA transfection?

After the transfection of my cell culture, I tried to measure the mRNA expression levels of a few genes including that targeted by my siRNA. All of them were decreased in the same pattern...

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