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I want to deliver viral vector to layer 2/3 in mouse cortex. What is the DV coordinate recommended and where the depth count start from ? is it the skull surface itself or the cortex surface?
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We would like to deliver DREADDs to different cortex layers and we think of using Cre- dependent DREADDs. So, is there transgenic mice which limit the expression of our DREADDs to Cortex layer 2/3...
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