Does anyone have advice on semi-random site-saturated mutagenesis?

I am trying to make a 51 or 36 base pair stretch of semi-random mutagenesis. I have made a forward primer with the generic sequence (for the 51 bp mutation): 5’-X(8)-N(51)-Y(20)-3’ Where X(8) is...

10 October 2013 2,682 8 View