Wajd Amly

6 Questions 3 Answers 0 Followers

Questions related from Wajd Amly

What does it means to have an elevated threshold like in the attached pic?

What does it means to have an elevated threshold like in the attached pic? The qpcr results for this gene gives me this curves. Other genes showed the normal curves. What is this means and does it...

03 March 2016 7,372 3 View

What does it mean to have N/A results in rt pcr for gDNA?

I performed a rtpcr run forr my cDNA but the results were N/A. I suspected that the cDNA might be damaged or has something wrong with it, therefore, I made new cDNA from my stored RNA. Following...

12 December 2015 1,259 0 View

Does entering wrong information in rt pcr machine affects the results?

I wonder if entering a wrong information to rtpcr machine would really affect the results. For instance, wrong concentration or dilution factor when labeling the plate before the run.???

12 December 2015 3,184 1 View

Why do I get double bands on gel electrophoresis for rtpcr standards?

I'm running a rtpcr to obtain the standard curve, however, I got 2 bands on gel electrophoresis for the NTC and the samples. I repeat the test 3 times, I got the same result in the first and...

11 November 2015 975 2 View

How can I distinguish between Primer dimer vs non specific amplification?

How can we distinguish between Primer dimer and non specific amplification using gel electrophoresis and melt curve?

11 November 2015 1,720 3 View

Does rtpcr results influnced by the cdna concentration and 260/280 ratio?

I have cDNA samples with concentrations range from 99- 6700 ng/ul and 260/280 ratio of 1.5- 2.02. I run a rtpcr using some samples to conduct the standard curve, however, the -ve (blank) curve was...

10 October 2015 7,829 3 View