Wajd Amly

6 Questions 2 Answers 0 Followers

Questions related from Wajd Amly

What does it means to have an elevated threshold like in the attached pic?

What does it means to have an elevated threshold like in the attached pic? The qpcr results for this gene gives me this curves. Other genes showed the normal curves. What is this means and does it...

23 March 2016 8,160 3 View

Does entering wrong information in rt pcr machine affects the results?

I wonder if entering a wrong information to rtpcr machine would really affect the results. For instance, wrong concentration or dilution factor when labeling the plate before the run.???

24 December 2015 790 1 View

When should the extracted rna be converted to cDNA?

what is the best time to convert rna into cdna? I have extracted my rna using trizol, stored it in -80 for maybe a month- month and a half before I synthesized my cdna. Does it affect my cdna and...

16 December 2015 5,927 2 View

Can I use Evagreen dye instead if Sybrgreen without any modification?

Can I use Evagreen dye instead if Sybrgreen without any modification? Does it has the same wavelength?

21 November 2015 2,906 2 View

Why do I get double bands on gel electrophoresis for rtpcr standards?

I'm running a rtpcr to obtain the standard curve, however, I got 2 bands on gel electrophoresis for the NTC and the samples. I repeat the test 3 times, I got the same result in the first and...

18 November 2015 4,245 2 View

Does rtpcr results influnced by the cdna concentration and 260/280 ratio?

I have cDNA samples with concentrations range from 99- 6700 ng/ul and 260/280 ratio of 1.5- 2.02. I run a rtpcr using some samples to conduct the standard curve, however, the -ve (blank) curve was...

15 October 2015 7,899 3 View