Wajd Alali

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Questions related from Wajd Alali

Why is TFA commonly used in LCMS sample preparation?

I plan to use saliva samples for LC-MS peptide analysis and noticed many papers treat them with 0.2% TFA in a 1:1 ratio. Why is TFA used, and does it solubilize / denature proteins? I know that...

25 September 2024 6,244 3 View

In the BCA Protein Assay, what is the Compatibility Reagent (Reducing Agent)?

I’m using the Pierce® BCA Protein Assay Kit − Reducing Agent Compatible from Thermo Scientific, and I have a few questions. Could you please refer to the protocol and help clarify the following:...

11 September 2024 777 2 View