Wai Ling Lai

4 Questions 5 Answers 0 Followers

Questions related from Wai Ling Lai

How to process NGS sequencing data for amplicons from multiplex?

Hi all, I've run 6 rounds multiplex PCR of 21 self-designed microsatellite primers on bear samples. I have pooled all the pcr products, purified the amplicons and sent for NGS sequencing. I have...

13 September 2017 8,429 2 View

How to use QDD on windows to design primers?

Dear all, I'm currently working on mammal genomes. I had received Illumina NGS data and decided to use QDD (on Windows) to fish out microsatellites for PCR primer design. I've encountered some...

15 January 2017 7,127 2 View

How to perform mitogenome assembly using data from Illumina Miseq NGS?

My NGS result is in "fastq.gz" format. is there any recommendations of softwares or websites I could use to process the data and hence assemble the mitogenome?

11 October 2016 5,435 3 View

Why there is no bands / smearing observed for genomic DNA samples during gel electrophoresis?

Sample: muscle tissue (~4mg) Extraction Kit: Macheney-Nagel - DNA, RNA & Protein Purification Kit DNA concentration (Biodrop) = ~20ng/µl Gel: 1% Agarose, 100V, 40minutes DNA ladder: 1kbp...

06 September 2016 1,596 13 View