The PK-15 cells are not detaching from the flask after trypsin treatment?

When I recover the PK-15 cells from the Liquid nitrogen after 48h I passage it, I use 1.5mL trypsin for 5 minutes at 37C to detach them. They detach very well but when I passage it on 2nd, 3rd and...

28 March 2019 4,436 3 View