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Hi all, I want to make the truncation of my gene, I divided the full length gene sequence into 7 parts (truncations), which is shown in the picture below. Is there any way or website to determine...
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When I recover the PK-15 cells from the Liquid nitrogen after 48h I passage it, I use 1.5mL trypsin for 5 minutes at 37C to detach them. They detach very well but when I passage it on 2nd, 3rd and...
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