Double digest for screening CYP2D6 using pGEX-6P-2

i did cloning for cytochrome P450- 2D6 into pGEX-6P-2 plasmid vector. when i screen using PCR i get the band of PCR product at an actual size of 1375 bp. but when i use double digest i do get...

30 January 2017 4,763 3 View