Vladislav Chalapa

4 Questions 6 Answers 0 Followers

Questions related from Vladislav Chalapa

How to optimize multiplex qPCR assay where two combined targets work poorly?

Dear researchers, I’m working on an Norovirus GI and GII qPCR assay with TaqMan probes. Recently, I experienced an issue with “multiplexing”. Briefly, the aim is to develop an assay for...

13 March 2024 4,879 0 View

Appropriate goodness-of-fit test for negative binomial regression?

I'm fitting a model in R where response variable is an over-dispersed count data, and where I meet an issue with goodness-of-fit test. As it has been previously discussed in Cross Validated,...

18 May 2023 2,194 1 View

How to draw vertical line on drapery plot in R (package meta)?

Dear researchers! I'm drawing drapery plot using meta package in R. I would like to add vertical line to show point estimate for odds ratio. How to get this using drapery function?

28 January 2022 3,199 3 View

What causes unrecognized peaks in Sanger sequencing chromatograms (Honor 1616 Genetic Analyser)?

Dear researchers! Me and my colleagues have only basic experience in Sanger sequencing. Recently we sequenced tick-borne encephalitis virus (TBEV) using Honor 1616 Genetic Analyzer (Nanjing...

09 January 2022 9,260 6 View