Vladimir Gritsenko

2 Questions 2 Answers 0 Followers

Questions related from Vladimir Gritsenko

Can PCR be used immediately after BAL 31 digestion, and if so, what is the best way?

Hello, First, let me describe the problem I'm trying to solve. I transformed yeast with an antibiotic marker to truncate a chromosome, with the fragment having a homologous sequence on one and...

06 August 2023 3,307 2 View

How to improve a multiplex PCR that's missing a few expected products (from mixed genomic and mitochondrial DNA template)?

Hello, I'm using the QIAGEN Multiplex PCR Kit and 10 primer pairs (product sizes from ~130 to ~900 bps) to monitor mitochondrial DNA in Candida glabrata, a close relative of Saccharomyces...

06 January 2022 8,965 3 View