How do you perform Immunofluorescence staining on thick brain slices (around 400 micrometers)? cut and then stain or whole mount stain and then cut?

Dear collegues, how do you would perform Immunofluorescence staining on thick brain slices (around 400 micrometres) upon acute slices pharmacological manipulation or electrophysiology? -cut and...

11 March 2021 1,671 2 View