Can i use primers from a part of intron to the contiguous exon to get information about primary transcript (pre-mRNA) of a specific gene by RT-qPCR?

Hi everyone, im interested into knowing the quantity of spliced and unspliced mRNA for a paticular gene. I thought using primers for mature mRNA (exon to exon) and other set of primers to quantify...

28 April 2022 6,952 2 View