Vivian Rincon-Florez

2 Questions 2 Answers 0 Followers

Questions related from Vivian Rincon-Florez

What's the difference between the Query covery in Geneios R10 and NCBI?

I have used the Geneiuos tool to BLAST my sequence and obtained a %pairwise identity and the query coverage of my sequence. However, if I take the same sequence and run a BLAST in the NCBI...

11 November 2017 9,990 2 View

Is it normal to obtain multiple bands for AmoA primers in soils?

I'm using Amoa_2R and Amoa_1F primers. After the PCR my gel showed multiple bands for the soil sample that I'm working with. I was expecting only a 481 bp. I tried different annealing temperatures...

05 May 2015 5,694 4 View