Vivian Chua

2 Questions 5 Answers 0 Followers

Questions related from Vivian Chua

Why is the amperage/current so low when using 'homemade' consumables on the transblot turbo?

We transferred proteins from SDS-PAGE onto nitrocellulose using standard homemade Towbin/transfer buffer (25mM Tris, 192mM, 20% methanol) and whatmann filter papers. The problem is that compared...

04 April 2014 6,126 3 View

Why are my real time PCR amplification curves jagged throughout despite neat and specific corresponding melting curves?

I used the Roche LightCycler 480 and MCF-7 breast cancer cell line cDNA samples to construct an efficiency curve for the ABCG2 gene. This is the 2nd repeat of the experiment and although my...

04 April 2012 9,638 22 View