Vitalijs Ovcinnikovs

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Questions related from Vitalijs Ovcinnikovs

Issue with tissue sectioning after PFA/Sucrose fixation?

As part of my recent work I had to resolve to PFA fixation followed by sucrose cryoprotection to preserve GFP in spleen tissue sections for IHC. However, i encountered an issue, where tissue...

09 September 2017 1,496 12 View

Problem with tissue sectioning after PFA/Sucrose fixation?

As part of my recent work I had to resolve to PFA fixation followed by sucrose cryoprotection to preserve GFP in spleen tissue sections for IHC. However, i encountered an issue, where tissue...

09 September 2017 2,809 0 View

Do human CMV and ubiquitin promoters express well in mouse bone marrow cells?

Hi, community! As part of my PhD project I have been trying to transduce mouse HSCs (Lin- or 5-FU treated Bone Marrow cells) with a lentivirus for subsequent transplantation and in vivo expression...

02 February 2016 3,709 1 View

What is the optimal cytokine mix for Lentiviral transduction of mouse Bone Marrow?

Hi science, As part of my PhD I need to perform transduction of RAG-/- mouse bone marrow with lentivirus for subsequent transplantation and with the goal of obtaining a transgenic population of...

06 June 2015 2,581 4 View