Vishnutej Ellur

3 Questions 4 Answers 0 Followers

Questions related from Vishnutej Ellur

I need some clarification regarding on inter-run calibration in qPCR?

Hello, I looking into relative expression of a gene at treated and untreated samples at 6 time points. All the samples with replicates does not fit in one plate. I have the following...

28 June 2019 2,915 7 View

How to avoid bursting of Eppendorf tubes after snap freezing in liquid nitrogen ?

Hello, I am collecting plant leaf and stem samples for RNA extraction for my RT-qPCR experiment. I snap freeze the samples/tissues in 2.0/1.7mL Eppendorf tubes in liquid nitrogen. During transfer...

04 June 2019 3,263 4 View

How to design primers that spans intron-exon boundaries?

Hello, I am doing a RT-qPCR and need to design a primer that spans intron-exon boundaries. I am working with chickpea plant. Can anyone of you help with the method of designing this? Here are the...

29 May 2019 1,569 2 View