PCR in the purified plasmid after cloning 2kb insert gene gives a result of 800bp. Test digestion shows no insert in the plasmid. What went wrong?

I am trying to clone a gene of 2kb size from one plasmid to another. I first did a pcr for my insert gene from plasmid 1 and then gel elution of amplified product. I then did a single digestion in...

01 January 2016 9,783 5 View