Vishnu Dutt

3 Questions 3 Answers 0 Followers

Questions related from Vishnu Dutt

Why got population shift in flow cytometer?

Hello connection While performing potency test by using BD FACS instrument some time we few population inside the gate few outside of gate and some time I got population outside of the gate I am...

18 March 2024 3,482 4 View

Why I am not able to isolate the plasmid after get colony PCR postive?

Hello everyone, In my case, i ligated my insert (804 bp) into pET30a expression vector (5422bp) 3:1 ratio containing 20 ul reaction by using T4 DNA ligase and incubated at 24 degree centigrade...

17 May 2023 849 3 View

Why my cloning are not working ?

Hello everyone, I have one doubt regarding cloning ? I am using pET30a (+) vector for cloning (5422bp) and Insert size 804bp. I tried all the ligation reaction ratio lilke 1:3, 1:5, 1;7 (100ng...

27 March 2023 6,997 6 View