Vishnu Dutt

2 Questions 1 Answers 0 Followers

Questions related from Vishnu Dutt

Why I am not able to isolate the plasmid after get colony PCR postive?

Hello everyone, In my case, i ligated my insert (804 bp) into pET30a expression vector (5422bp) 3:1 ratio containing 20 ul reaction by using T4 DNA ligase and incubated at 24 degree centigrade...

17 May 2023 833 3 View

Why my cloning are not working ?

Hello everyone, I have one doubt regarding cloning ? I am using pET30a (+) vector for cloning (5422bp) and Insert size 804bp. I tried all the ligation reaction ratio lilke 1:3, 1:5, 1;7 (100ng...

27 March 2023 6,986 6 View