Vishal Vasant Naik

5 Questions 6 Answers 0 Followers

Questions related from Vishal Vasant Naik

Does constituents of nitrogen metabolism have any role in plant stress tolerance?

e.g. nitrate, nitrite, nitrate reductase etc. I am working on effect of water stress and salinity on nitrogen metabolism of plants. I found that amino acids like proline has definite role in...

13 December 2014 6,839 4 View

Can I use Triton-X-100 instead of CHAPS in TCA-Acetone precipitation protocol for proteins?

Because both are detergents and are used in various biochemical assays. The precipitate formed due to TCA is soluble in standard sample solubilization buffer (8M Urea, 4% w/v CHAPS...

22 May 2014 6,617 3 View

How do I eliminate "Smiling Bands" of proteins in SDS-PAGE?

I am working on SDS-PAGE for proteins and observed 'smiling bands'. Is this happen due to temperature generated during electrophoresis?

25 April 2014 8,791 15 View

How do I extract proteins for an SDS-PAGE from mucilage rich plants?

I am working on a protein profile of Portulaca oleracea.

04 March 2014 6,797 11 View

Which criteria will be more suitable to compare methods?

I am working on the comparison of methods for biochemical analysis. I found that in some literature R² value from graph is used to decide the accuracy of the method. Is it the best way to compare...

01 October 2013 5,637 11 View