8 Questions 9 Answers 0 Followers
Questions related from Virginie Mournetas
When I test whether my qPCR data are normally distributed with a Shapiro-Wilk test (using SPSS), I sometimes find that they do NOT follow a normal distribution. Do you have this problem? What do...
12 December 2014 3,071 11 View
Dear all, I would like to know: Is GSEA (http://www.broadinstitute.org/gsea) better than DAVID (http://david.abcc.ncifcrf.gov/) as an enrichment tool? Which one do you recommend and why? Would it...
09 September 2014 3,783 4 View
I am wondering if this iBind™ Western Device from Life Technologies (https://www.lifetechnologies.com/order/catalog/product/SLF1000?ICID=search-product) is worth buying or not. I am looking for...
05 May 2014 164 1 View
I would like to perform Western Blot with proteins which are secreted. One of them is 45 kDa and the other is 30 kDa. In one condition, I have BSA added to the cell culture medium, together with...
09 September 2013 1,567 20 View
I found by chance a text about antibody blocking peptide which seems to be used to test the specificity of primary antibodies. I was wondering if someone has experience with this technique?
04 April 2013 733 1 View
I am currently doing qPCR using SYBR detection. When I want to assess the amplification efficiency, sometimes I get more than 100% and I don't understand why. Moreover, people say that it is not...
08 August 2012 8,073 16 View
In terms of results and efficiency, can we find differences between classical PCR such as Enzyme Activation: 95°C 10 min PCR (40 cycles): 95°C 15 sec + 60°C 1 min and a fast PCR? Enzyme...
04 April 2012 9,872 10 View
I would like to know if the old fashion protocol (cell lysis, RNA extraction, RT and then qPCR) is better than kits such as "Power SYBR® Green Cells-to-CT™" from Life Technologies which permit you...
04 April 2012 8,082 34 View
