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Questions related from Virginie Mournetas
Dear all, I would like to know: Is GSEA (http://www.broadinstitute.org/gsea) better than DAVID (http://david.abcc.ncifcrf.gov/) as an enrichment tool? Which one do you recommend and why? Would it...
10 September 2014 2,401 3 View
I am trying to quantify produced pseudotyped lentiviruses. I read that the HIV virus has 2 copies of its RNA genome. Is ths still true for "home-made" pseudotyped lentiviruses? Thank you for your...
31 May 2014 2,854 2 View
I would like to perform Western Blot with proteins which are secreted. One of them is 45 kDa and the other is 30 kDa. In one condition, I have BSA added to the cell culture medium, together with...
18 September 2013 1,997 21 View
As it is relatively hard to publish negative results, I have trouble finding answers to my question. I know there are many studies showing the induction of pluripotency from human somatic cells by...
28 March 2013 5,936 0 View
I will use lentivirus soon to integrate an shRNA in my cells. Unfortunately, the shRNA vector does not carry a fluorescent protein. How can I determine the titration of my lentivirus?
20 July 2012 7,108 11 View
I would like to know if the old fashion protocol (cell lysis, RNA extraction, RT and then qPCR) is better than kits such as "Power SYBR® Green Cells-to-CT™" from Life Technologies which permit you...
22 April 2012 912 32 View
In terms of results and efficiency, can we find differences between classical PCR such as Enzyme Activation: 95°C 10 min PCR (40 cycles): 95°C 15 sec + 60°C 1 min and a fast PCR? Enzyme...
22 April 2012 386 9 View
