Virginia Morón-Calvente

2 Questions 2 Answers 0 Followers

Questions related from Virginia Morón-Calvente

I recently started to work with THP-1 cells, I need to differentiate them to macrophages using PMA. They are dying. Why is this happening?

I have THP-1 on an early pass (they are 5th pass). So I bought 5mg of PMA (sigma), and diluted it in 1mL of DMSO. I tried 3 different concentrations to differentiate the cells: 10ng, 50ng and...

18 February 2021 1,710 5 View

Which are the best differentiation markers in Thp-1 and U937 cells?

I use PMA to differentiate thp-1 and u937 cells to macrophage. I usually use CD11b as differentiation marker but I need another one.

27 August 2014 737 7 View