Virginia Aragon

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Questions related from Virginia Aragon

Why are most people doing TFM using gels of stiffness in the KPa range? Stiffness of BM measured by ATM is in the MPa range. What am I missing here?

I am designing experiments to measure contractile forces of endothelial cells in response to certain stimuli. To start I have been reading a lot about Traction Force Microscopy (TFM) and noticed...

08 September 2015 498 0 View

Can multiple radio-labeled probes be used at the same time for PET imaging?

If different isotopes (11-C, 89-Zr, 64Cu) are used to label different antibodies, can they all be used at the same time during PET imaging? Or because all of them undergo Beta+ decay and produce...

22 May 2014 7,737 9 View

Can someone advise on Affinity vs. Dynamic Affinity?

I have been reading some papers regarding the dynamics of receptor-ligand binding for my project and have come across this term "dynamic affinity" quite often and I am wondering if it actually has...

18 October 2013 6,580 2 View

Microrheology experiment design - can anyone help?

Does anyone know how to determine what the correct bead size for a microrheology experiment should be?

14 October 2013 8,322 3 View

Any suggestions for establishing a Tetracycline inducible mammalian cell line using the TRex system from Invitrogen?

For the last couple of months I have been trying to establish an inducible HaCaT cell line using the TRex system from Invitrogen. I am following the standard protocol (first transfect with...

29 January 2013 5,259 15 View