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Questions related from Vipul Kumar
Which mutation is least affecting to the stability of protein if is to be done on surface. If Ala(on surface, with methyl group facing outwards) is mutated to Cys, would it affect the stability?...
18 March 2016 8,926 6 View
As the enzyme contains Mg2+ion bound in the native state, the buffer used for its assay also contains 10 mM MgSO4. I read somewhere that the buffer should contain EDTA and no reducing agent and...
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If I load high amount of protein on SDS PAGE and do densitometric analysis (I am using BIO RAD's Image Lab tool) the relative intensity/amount of other bands increases to higher extent than the...
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The query is that when I initially performed RT-PCR with my specific primer at a given set of conditions, good results were obtained (i.e no primer dimer formation observed when checked through...
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