Has anyone ever found MMP-10 or other MMPs with a much greater molecular weight with western blotting?

I am performing some WBs to detect MMP-10 in mouse skeletal muscle tissue. MMP-10 as zymogen is ~54KDa and when active is ~45KDa, but all my samples show one single clear band of ~85KDa. The...

03 March 2014 6,589 4 View