Vinny Vinod

2 Questions 6 Answers 0 Followers

Questions related from Vinny Vinod

How to concentrate the protein after fermentation using Pichia pastoris?

After 4 Times running the fermenter and optimising each time I have got my protein(pPIC9K VECTOR) the fourth time in the methanol fed batch phase within 48 hours . If I am keeping for more time I...

02 February 2020 2,132 2 View

How can I confirm protein expression from Pichia pastoris colonies?

I was successful with the transformation( pPIC9K vector in Pichia pastoris GS115 strain )and got good colonies in RDB plates after 48 hours. later in MD and MM plates also. Can anyone please tell...

12 December 2019 8,917 8 View