Vini Gautam

3 Questions 15 Answers 0 Followers

Questions related from Vini Gautam

What are the reasons for dead cells in primary hippocampal culture?

I have been trying to grow primary culture from isolated hippocampi from rat pups. Sometimes the cells survive while most other times they strangely land up dead like debris after 3-4 days. I have...

08 October 2015 2,089 11 View

What might cause cells to "burst" after taking them out of the incubator?

I have been growing neuroblastoma B50 cells on glass coverslips and the cells appear healthy when I see them under the normal microscope. For electrophysiology, I take the coverslip out and place...

10 February 2015 9,653 3 View

Do neuronal cell lines exhibit action potentials?

I have recently started to work with B50 neuronal cell line. The cells grow and proliferate well, but they do not exhibit any signals when I try to record action potentials from them using patch...

08 January 2015 4,543 10 View