Vineeta Sharma

4 Questions 5 Answers 0 Followers

Questions related from Vineeta Sharma

Anyone knows Commercial source of HUDEP-2 Cell Lines ?

Does anyone know from where to get HUDEP-2 cells?

03 March 2016 1,147 7 View

CD34+ Cell culture condition?

Could you please share a protocol for culture and expansion of CD34 cells? Also, how to keep them in culture for 10-14 days without differentiation? Thanks!

03 March 2016 1,735 4 View

How to Deal with ELISA High Background Issue?

I am trying to develop an ELISA to measure Ab against aden associated virus (AAV). I am using Nunc-Immuno™ MicroWell™ 96 well solid plates (Sigma-Aldrich; Cat.# M9410-1CS) to coat AAV using...

02 February 2016 9,522 6 View

Roller bottle cell culture conditions?

I am planning to grow HEK293 into 1700 cm2 roller bottles. Could anyone please share a protocol to grow cells in rollers (details about the speed, volume and other parameters)? Thanks a lot.

12 December 2015 5,670 2 View