Why have I a presence of a miRNA in Jurkat cells that I have specifically mutated, insering a donor DNA?

Hello, I have a strange problem. In my lab we are trying to use CRISPR/Cas9 technique to create a Knock-out of a miRNA in Jurkat cells ( T cells) . In detail I used a crRNA that target...

02 February 2018 5,301 0 View