ELISA can't detect RANKL in MC3T3 supernatant but cell lysate is ok, does anyone have similar experience?

I tried to measure cell lysates and cell culture supernatants in differentiated MC3T3 using mouse RANKL ELISA kit (#EMTNFSF11, Thermofisher). While the cell lysates gave me a consistent reading...

06 June 2018 6,566 2 View