Vinay Bachu

4 Questions 7 Answers 0 Followers

Questions related from Vinay Bachu

Why no protein expression in BL21 DE3?

We have purchased an expression vector from DNASU (pANT7_cGST). The expression vector has a human protein cDNA as insert. we have made multiple attempts to express the protein in different con of...

09 December 2019 3,109 8 View

Effect of centrifugation on DNA-protein complex?

Will there be a separation of protein and DNA on centrifugation? Lets consider a scenario where in, DNA interacts with protein and forms a stable complex, will the complex break on centrifugation?...

29 January 2019 7,114 4 View

Why is PEO dissolved solution in water white?

I am trying to dissolve PEO of mol. weight 1,00,000 in water and I am trying to prepare a 30% W/W solution. I sucessfully dissolved PEO in water, but the solution formed is not transparent and is...

22 December 2015 2,711 1 View

Which are the Dyes which can bind specifically to ssDNA and do not bind dsDNA?

We want to differentiate the ssDNA and dsDNA in the solution and the simple absorption spectrum at 260/280 fails to give us the individual values. Hence we want to use organic dyes which are not...

01 January 1970 9,710 4 View