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Questions related from Vikas Kukreja
Does the E.coli DH5 alpha have an inherent ligase? If yes, is it possible that a restricted digested plasmid would get recircularized using the ligase from the E.coli DH5 alpha once its...
07 July 2014 9,959 3 View
I wanted to screen for an antibiotic binding protein (aminoglycoside phosphotransferase). So the idea is to run a native PAGE of the crude protein extract and then incubate it with the antibiotic...
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