Vikas K Singh

5 Questions 10 Answers 0 Followers

Questions related from Vikas K Singh

Is it acceptable to mix splenocytes isolated from two different mice immunized with same antigen?

I want to perform splenocyte restimulation culture for the estimation of Th1 and Th2 cytokines by ELISA. As each groups consists of 5 mice immunized with a test vaccine preparation; can splenocyte...

09 September 2019 4,650 2 View

Is it possible do ammonium sulphate fractionation/precipitation of a protein inclusion bodies (IB's) dissolved in 8M Urea?

My protein (64kDa) expressed in insoluble inclusion bodies. I tried to purify it by ion-exchange and affinity but unable to purify protein. So, want to try ammonium sulfate precipitation of...

03 March 2018 8,305 5 View

Why are so many band in Western blot?

Western of His-tagged protein.

02 February 2018 9,961 0 View

How to avoid primer dimer in PCR?

I want to amplify a gene whose end terminus sequence is in such a way that the after designing NotI restriction site inserting (reverse) primer; it forms primer dimer during PCR amplification. I...

09 September 2017 7,888 3 View

What cause up shift of protein band in SDS-PAGE?

I am working on recombinant protein (Ig's) expressed in form of inclusion bodies. Upon giving certain treatments there is a up shift in protein band. If my protein is degrading during the process;...

11 November 2016 7,838 4 View