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Questions related from Vikas K Singh
I want to perform splenocyte restimulation culture for the estimation of Th1 and Th2 cytokines by ELISA. As each groups consists of 5 mice immunized with a test vaccine preparation; can splenocyte...
09 September 2019 4,671 2 View
My protein (64kDa) expressed in insoluble inclusion bodies. I tried to purify it by ion-exchange and affinity but unable to purify protein. So, want to try ammonium sulfate precipitation of...
03 March 2018 8,321 5 View
Western of His-tagged protein.
02 February 2018 9,978 0 View
I want to amplify a gene whose end terminus sequence is in such a way that the after designing NotI restriction site inserting (reverse) primer; it forms primer dimer during PCR amplification. I...
09 September 2017 7,913 3 View
I am working on recombinant protein (Ig's) expressed in form of inclusion bodies. Upon giving certain treatments there is a up shift in protein band. If my protein is degrading during the process;...
11 November 2016 7,861 4 View