Vikas Verma

3 Questions 5 Answers 0 Followers

Questions related from Vikas Verma

Regarding PBMC isolation using Histopaque 1119 and 1077?

Hi, I am isolating PBMC from undiluted human blood using histopaque 1119 and 1077(Sigma). In our system, we can not dilute the blood because simultaneously we need plasma in original...

21 January 2020 3,974 1 View

What is Standard reagent for WST8 validation?

I need ti validate WST8 assay in my lab. So could anyone please let me know what standard solution or QC I can use for the same for my future studies?

08 April 2019 3,399 4 View

While knockdown of specific gene by sh RNA, protein and RNA expression should be similar or may differ from each other?

Decrease in protein expression confirms the knockdown of specific gene, but what could be the reason if RNA level is not decreased? What are the ideal conditions? Should RNA and proteins be...

27 January 2016 7,315 5 View