Vikas Sharma

5 Questions 9 Answers 0 Followers

Questions related from Vikas Sharma

Can we amplify gene of interest from cDNA or mRNA using single degenerate primer?

I want to clone my gene of interest from a eukaryote.....I have a single 5' degenerate primer available to me. can I use single 5' degenerate primer from my amplification and oligo T as my reverse...

15 February 2016 9,845 3 View

Do we have to add extra base in front of ATG of the desired protein when cloning in BamHI site of pET22b?

I have cloned a PCR product into BamHI, Not1 site of pET22b but I am not seeing the protein. Do I have to add an extra base in front of ATG of my gene in order to make it in frame or can I use...

03 December 2014 215 2 View

Can anyone suggest the endochitinase enzyme activity assay from paenibacillus strain?

I have zone of clearance on Collidal chintin plate but I am not getting enzyme activity when inoculated from broth. I am using DMAB method. Is there different assay methods to differentiate...

21 November 2014 814 5 View

Can anyone suggest as to why I am not able to see the expression of 45KDa protein in pET22b?

I have cloned my genes of interest in pET22b and still not able to see the expression in BL21(De3)plys.

20 November 2014 6,338 7 View

How can I detect siderophore production on a solid agar plate?

Hi..I would like to detect siderophore production by rhizobacteria. I have rhizosphere soil samples to test and have used a CAS overlay method that hasn't worked since the original colour was red...

16 July 2014 6,971 5 View