Vikas Kumar Patel

4 Questions 26 Answers 0 Followers

Questions related from Vikas Kumar Patel

Can anyone suggest me a manual protocol for DNA purification from agarose gel?

I am trying to elute a DNA band from agarose gel using the manual protocol; in this I cut the DNA band from the gel on the transilluminator (tried to expose to UV light for very short duration),...

19 January 2014 6,424 41 View

May i use the SALTS OF Ni2+ at the place of Ni2+ for inducing the promoter activity

I am using a inducible expression vector for expression of gene. The promoter in this vector will induce in the presence of 5 uM Ni2+. I have salts of Ni2+, so will nickel salt work?

29 August 2013 7,453 1 View

Can anyone suggest to me why I am not getting amplification of the gene?

I am trying to amplify a gene 1.2 kb by using the self-designed forward and reverse primers (by using OLIGOS SOFTWARE) having the Tm respectively of 61.3 C and 63.0 C. The isolated genomic DNA...

14 August 2013 6,157 27 View

How to develop the competency in filamentous organism?

How can we develop the competency in filamentous organism like Nostoc and Anabaena spp.?

19 December 2012 6,714 3 View