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Questions related from Vikas K Singh
My protein (64kDa) expressed in insoluble inclusion bodies. I tried to purify it by ion-exchange and affinity but unable to purify protein. So, want to try ammonium sulfate precipitation of...
07 March 2018 693 4 View
Upon induction with IPTG in TB media, cells turned into pinkish color and yielded brownish inclusion body; while same BL21 culture inoculated in LB media resulting in green color. I have been...
07 June 2017 2,057 5 View
I am unable to make BL21 DE3 competent cells. I have tried many protocols including CaCl2, MgCl2, and TSS method but every time I failed. On the other hand, if I prepare competent cells for DH5a...
30 April 2017 4,107 2 View
I am working on recombinant protein (Ig's) expressed in form of inclusion bodies. Upon giving certain treatments there is a up shift in protein band. If my protein is degrading during the process;...
20 November 2016 1,882 3 View
As we know final concentration of dNTP's in PCR reaction is 0.2mM and during replication intracellular concentration of rNTPs is 10–100-fold or more higher than that of dNTPs. But what is the...
11 March 2016 1,469 1 View