Vijitra Luang-In

5 Questions 5 Answers 0 Followers

Questions related from Vijitra Luang-In

Why use V3-region of the 16S rRNA encoding gene for bacterial sequencing?

I have always come across ''Changes in bacterial composition were determined using high-throughput sequencing of the V3/V4-region of the 16S rRNA encoding gene''. Could anyone explain to me why...

27 July 2019 9,907 5 View

Which dialysis tubing molecular weight cut off (MWCO) should I use for bacterial exopolysaccharide?

I have exopolysaccharide (EPS) extracted from Bacillus sp. and wanted to dialyze it overnight. From previous papers they suggested 8,000 - 14,000 Da, 12,000-14,000 Da and 3,500-4,000 Da Molecular...

24 February 2016 4,279 0 View

How to solve low % Bootstrap on the phylogenetic tree?

I am new to this and I have just constructed a phylogenetic tree of 24 bacteria isolated from a kefir generated from the PCR-amplified bacterial 16S rDNA partial sequences (ca. 900 bp) based on...

17 February 2016 10,007 5 View

What to do if bacterial strain identification by 16S rDNA sequence of unknown bacteria does not match its Gram staining result?

We isolated a coccus, Gram-negative bacterium from soil and identified the species by 16S rDNA gene analysis. The 16S rDNA is of 1,498 bp. Surprisingly we got the isolate identified as Bacillus...

18 December 2015 1,767 4 View

In Gram staining, what are the tiny dark purple dots inside or outside the bacterial cells?

Can anyone please view the attached pictures and tell me what are tiny dots inside or outside the bacterial cells that have been stained dark purple as opposed to lighter purple cells? Cells are...

24 November 2015 7,058 16 View