Vijayakumar Rajendran

4 Questions 33 Answers 0 Followers

Questions related from Vijayakumar Rajendran

Why I am getting a peak at ~380nm in Tryptophan Fluorescence?

I did Tryptophan quenching studies of my truncated protein excitted at 295nm with the emmission range 300-500 nm. I am getting two peaks one at 335nm (one for tryptophan) and other at 380nm. The...

11 April 2017 3,757 24 View

What are the steps to purify the occluded his tag recombinant protein?

My recombinant protein of 38 kDa has been expressed and it was in soluble fraction (with 10% glycerol, 0.5mM IPTG and 23 degree celcius). but the protein was not binding to the Ni-NTA column. I...

10 June 2016 764 13 View

How can I cross link proteins using glutaraldehyde?

My recombinant protein is a homodimer whose monomer is ~61Kda. I tried to crosslink the protein with 1%, 2.3% of glutaraldehyde with various durations from 2 minutes to 30 minutes. But none of the...

12 August 2015 4,927 17 View

Does anyone have experience with protein expression shows 20Kd instead of 60Kd in SDS?

I cloned my protein, its size is approximately 60Kd. But its expression shows it was just 20Kd approximately. If there is a possibility of frame shift mutation, can I rectify it by changing the...

13 June 2014 9,854 2 View