Vijaya Sarathy

2 Questions 9 Answers 0 Followers

Questions related from Vijaya Sarathy

Can anyone suggest the recommended buffer for incubating enzymatic cell free extract for 48hrs?

I am going to assess the ability of Cell free extract(taken from potential bacterial cells) to degrade the aromatic substance. So for checking the degradation activity i need to incubate the cell...

14 April 2018 2,934 2 View

Can anyone guide me how to get maximum of total protein from the bacterial cell. Suggest suitable lysis buffer composition applid before sonication?

I have gone through sonication process for bacterial cells( treated as wells as control ) to estimate total protein. For that 500ml of log phase cultures harvested, washed and concentrated using...

28 March 2018 9,918 13 View