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Questions related from Vijay Barathi Elango
I have attached a couple of images (cells in a hemocytometer under a cover slip). Is the structure a protoplast ? If yes, do protoplasts have non-spherical structures ? ( excuse the poor image...
24 January 2020 9,468 5 View
I am new to plant biology, looking for a gRNA and a Cas9 expressing construct to transform dicot plants. pRGE31 hosts a gRNA scaffold sequence but can't find a restriction site upstream of the...
10 December 2019 3,402 3 View
I have been transfecting Plat-E cells ( encodes gag-pol, env genes ) with pMX-dsRed which contains the 'psi packaging signal' Starting population : 6x10^cells (PlatE) - one 10cm dish pMX-dsRed...
05 October 2017 1,652 5 View
Does the presence of an attR1 sequence (~ 120bp) in between a 3xFlag sequence and the N-terminal of gene of interest affect translation of the gene. The backbone destination vector has the 3X Flag...
23 November 2016 2,072 2 View
