What is the best method to avoid freezing artifacts in skeletal muscle? I am following the protocol of isopentane in liquid nitrgoen?

Ø Before starting the muscle biopsy procedure, pour 100 mL (approximately 1/3 of beaker) isopentane (2-methybutane) to a glass beaker or a steel beaker. Ø Suspend the beaker in a bath of liquid...

07 October 2021 3,585 3 View