Vidya Rajasekaran

5 Questions 7 Answers 0 Followers

Questions related from Vidya Rajasekaran

What happens to the DNA from sloughed off intestine epithelial cells when extracting bacterial DNA from mouse fecal samples?

I am currently using the PowerSoil DNA extraction kit from MoBio for DNA extraction from bacteria present in mouse fecal matter. I wanted to know how the sloughed off intestine epithelial cells...

08 August 2016 1,472 1 View

Has anyone maintained APCmin/+ mice on RM1 (Company: Special Diets Services) maintenance diet instead of RM3 breeding diet?

Recently, there was a change in diet of the APCmin/+ mouse colony on B6 background. Instead of being fed the RM3 (highly nutritious) breeding diet, the APCmin/+ breeders were fed RM1 (low in...

03 March 2015 2,012 0 View

How can I prevent loss of tissue during H&E staining of cryostat section and what software can I use to quantify the size of polyps in the samples?

I am trying to perform H&E staining on mouse guts (Swiss Roll). I have fixed the guts in NBF at room temperature overnight before preparing the Swiss Rolls. The cryosections that I made seem to be...

03 March 2014 6,133 7 View

mRNA to protein expression?

I have been conducting a series of experiments where I transiently transfect plasmid with my gene of interest into HT29 cells. When I performed qRT-PCR on these cell samples, I can clearly see an...

12 December 2013 314 9 View

Can protein expression stop in cell lysates?

I collected whole cell lysates of HT29 Clones in RIPA buffer (Protease Inhibitor cocktail, PMSF, Na3VO4 and NaF all added into the RIPA buffer) and analyzed them by western blot on the same day...

11 November 2013 5,655 5 View